[BioC] regarding to flowCore/read.FCS warning message

Mike wjiang2 at fhcrc.org
Fri Oct 19 22:54:09 CEST 2012


This warning message is due to the fact that R doesn't handle unsigned 
integer of size larger(or equal) than 4 Bytes.
If your raw files store data as non-integer type (float or double),you 
can safely ignore this warning. Let me know if you have more questions 
regarding to flowCore.

Mike Jiang,PhD
System Analyst
PS Statistics, Vaccine and Infectious Disease Division
Fred Hutchinson Cancer Research Center

On 10/19/2012 12:01 PM, Stevan Lauriault wrote:
> Hi,
> I've been given the assignment of reviewing the flowCore package for 
> the core flow facility here in Toronto and I'm encountering a few issues.
> First, when calling the read.FCS function to read a flow file:
> STL1 <- read.FCS("/users/steve/desktop/AMLX/Specimen_002_CD3.fcs", 
> transformation = FALSE)
> the following message is returned:
> Warning message:
> In readBin(con = con, what = dattype, n = count, size = size, signed = 
> signed,  :
>   'signed = FALSE' is only valid for integers of sizes 1 and 2
> I've tried multiple files and the same message is returned.  Any ideas?
> Regards,

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