[BioC] readQpcrBatch function in qpcrNorm

Mueller, Alan A.J.Mueller at liverpool.ac.uk
Tue Sep 4 16:25:55 CEST 2012

Dear List, 

I am having difficulty reading in multiple text files using the 'readQpcrBatch' function from qpcrNorm.  Reading in single files is not a problem:

> data=readQpcr("file1.txt",header=FALSE)
> slotNames(data)
[1] "geneNames"  "plateIndex" "exprs"      "normalized" "normGenes" 

# However, trying to read all text files from an autonomous folder results in this error:

> data=readQpcrBatch()
Error in initialize(value, ...) : 
  no slot of name "primerNames" for this object of class "qpcrBatch"

#The columns conform to the requirements of readQpcr and I get this error even if I read in the data file 'qpcrBatch.object ' that is provided with the vignette as a text file.  
#The same outcome results from:

> data=readQpcrBatch(filenames=c("file1.txt","file2.txt"),header=FALSE)
Error in initialize(value, ...) : 
  no slot of name "primerNames" for this object of class "qpcrBatch"

> sessionInfo()
R version 2.15.1 (2012-06-22)
Platform: x86_64-pc-mingw32/x64 (64-bit)

[1] LC_COLLATE=English_United Kingdom.1252 
[2] LC_CTYPE=English_United Kingdom.1252   
[3] LC_MONETARY=English_United Kingdom.1252
[4] LC_NUMERIC=C                           
[5] LC_TIME=English_United Kingdom.1252    

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] qpcrNorm_1.14.0    affy_1.34.0        limma_3.12.1       Biobase_2.16.0    
[5] BiocGenerics_0.2.0

loaded via a namespace (and not attached):
[1] affyio_1.24.0         BiocInstaller_1.4.7   preprocessCore_1.18.0
[4] tools_2.15.1          zlibbioc_1.2.0       

Any suggestions are gratefully received.



Alan James Mueller BSc BVM&S MRes MRCVS

Room 1.02
Comparative Musculoskeletal Research Group
The School of Veterinary Science
University of Liverpool
Chester High Road
CH64 7TE 

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