[BioC] Depth of Coverage and Base counts from BAM file

[Julian Gehring]

Hi Chandu,

The 'applyPileups' in the 'Rsamtools' packages will help you here:

"An array of dimensions nucleotide x file x position.
The ‘nucleotide’ dimension is length 5, corresponding to ‘A’, ‘C’, ‘G’, 
‘T’, and ‘N’ respectively." (Taken from the 'Rsamtools' manual)

The coverage you can then get with a function like 'rowSums'.

Best wishes
Julian


On 08/29/2013 09:58 AM, Chandu [guest] wrote:
>
> Hi ALL,
>
> Given BAM file, I like to get output something like below. Any ideas?
>
>
> Chr     Position     DepthOfCoverage         BaseCounts
> chr1   14112	   14	                         	A:0 C:0 G:0 T:14 N:0
> chr1   14113	   13		                	A:0 C:13 G:0 T:0 N:0
> chr1   14114	   11		                        A:0 C:0 G:0 T:11 N:0
> chr1   14115	   11		                 	A:10 C:0 G:0 T:1 N:0
>
> Many thanks in advance.
>
> Best Regards
> Chandu
>
>   -- output of sessionInfo():
>
> R version 3.0.0 (2013-04-03)
> Platform: x86_64-apple-darwin10.8.0 (64-bit)
>
> locale:
> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
>
> attached base packages:
> [1] stats     graphics  grDevices utils     datasets  methods   base
>
> --
> Sent via the guest posting facility at bioconductor.org.
>
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