[BioC] Agilent Mouse 8x60K array
James W. MacDonald
jmacdon at uw.edu
Mon Feb 4 19:44:09 CET 2013
Hi Nat,
On 2/4/2013 9:47 AM, Nathan (Nat) Goodman wrote:
> Greetings- I have been unable to find a bioc package which does for the agilent Mouse 8x60K array, what the affy package does for affymetrix arrays. Any pointers?
These Agilent arrays have a single 60-mer per transcript, so don't
require something like the affy package (which is intended to summarize
multiple 25-mers for a transcript to a single statistic). Instead, you
most likely just need something like limma, which has the necessary
functionality to read the data in, read in the GAL file so you annotate
your output, normalize, and make comparisons.
The limma User's Guide has several Agilent examples, IIRC, so I would
start there.
Best,
Jim
>
> Many thanks,
> Nat Goodman
> ISB
>
>
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James W. MacDonald, M.S.
Biostatistician
University of Washington
Environmental and Occupational Health Sciences
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