[BioC] XPS: Arabidopsis Gene 1.0 ST Array, import.exon.scheme ERROR

Endo [guest] guest at bioconductor.org
Sat Feb 16 11:04:14 CET 2013


Hi, 

I have struggled with an error that I cannot quite figure out. 
I'm using 64-bit Mac OSX (10.7.5) and would like to analyze Arabidopsis Gene 1.0 ST chip data by using the XPS package.

I used import.exon.scheme as mentioned in the help files as follows:

> library(xps)
Welcome to xps version 1.18.1
    an R wrapper for XPS - eXpression Profiling System
    (c) Copyright 2001-2012 by Christian Stratowa
> libdir <- "~/desktop/workspace/libdir"
> anndir <- "~/desktop/workspace/anndir"
> scmdir <- "~/desktop/workspace/scmdir"
> scheme.aragene10stv1.na33 <- 
+ import.exon.scheme("Scheme_AraGene10stv1_na33", filedir = scmdir,
+    paste(libdir, "AraGene-1_0-st.clf",sep="/"),
+    paste(libdir, "AraGene-1_0-st.pgf",sep="/"),
+    paste(anndir, "AraGene-1_0-st-v1.na33.tair10.probeset.csv",sep="/"),
+    paste(anndir, "AraGene-1_0-st-v1.na33.tair10.transcript.csv",sep="/"))

I get the following Error:

 Error in import.exon.scheme("Scheme_AraGene10stv1_na32", filedir = scmdir,  :
  error in function ‘ImportExonSchemes’
Creating new file </Users/Endo/desktop/workspace/scmdir/Scheme_AraGene10stv1_na33.root>...
Importing </Users/Endo/desktop/workspace/libdir/AraGene-1_0-st.clf> as <AraGene-1_0-st.cxy>...
   <610001> recError: Premature end of file <~/desktop/workspace/anndir/AraGene-1_0-st-v1.na33.tair10.probeset.csv> reached.
Error: Missing probeset annotation tree <AraGene-1_0-st.anp>.
Error: Could not get tree <~/desktop/workspace/libdir/AraGene-1_0-st.pgf>.
   <1416100> records imported...Finishedworkspace/anndir/AraGene-1_0-st-v1.na33.tair10.probeset.csv> reached.
New dataset <AraGene-1_0-st> is added to Content...
Importing </Users/Endo/desktop/workspace/anndir/AraGene-1_0-st-v1.na33.tair10.probeset.csv> as <AraGene-1_0-st.anp>...
Importing </Users/Endo/desktop/workspace/libdir/AraGene-1_0-st.pgf> as <AraGene-1_0-st.scm>...

and then get stuck.
What's going on?
Any help would be very appreciated

Many thanks,

Endo Motomu

 -- output of sessionInfo(): 

R version 2.15.2 (2012-10-26)
Platform: x86_64-apple-darwin9.8.0/x86_64 (64-bit)

locale:
[1] ja_JP.UTF-8/ja_JP.UTF-8/ja_JP.UTF-8/C/ja_JP.UTF-8/ja_JP.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] xps_1.18.1

loaded via a namespace (and not attached):
[1] tools_2.15.2

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