[BioC] Regarding Limma differential expression.

[James W. MacDonald]

Without replicates all you can really do is compute fold changes. You 
might get some traction with the RankProd package, but I have no 
experience so can't say for sure.

Best,

Jim



On 2/19/2013 8:28 AM, nitin mandloi wrote:
> Dear sir,
>
>                 I am doing differential expression analysis with limma. In
> ebFit = eBayes(fit2) I am getting error:  Error in ebayes(fit = fit,
> proportion = proportion, stdev.coef.lim = stdev.coef.lim,  :  No residual
> degrees of freedom in linear model fits. It is may be because I don't have
> replicates. So what should I do to for differential expression now.
>
>
>
>
>
> Thank you
>
> Nitin Mandloi
>
>
>
> Sandor Proteomics Pvt. Ltd., hyderabad
>
>
> 	[[alternative HTML version deleted]]
>
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-- 
James W. MacDonald, M.S.
Biostatistician
University of Washington
Environmental and Occupational Health Sciences
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Seattle WA 98105-6099