[BioC] edgeR: counts or pseudo.counts?

Davide Cittaro cittaro.davide at hsr.it
Wed Feb 20 07:22:24 CET 2013


Hello list,
I'm trying to use read counts normalized with edgeR outside R. After a DGEList has been normalized by TMM and dispersion has been estimate (at least with estimateCommonDisp) I have at least three ways to export data:
cpm(y)
cpm(y, normalized.lib.size=T)
y$pseudo.counts

Which would be the most appropriate? Actually I'm having really good results with pseudo.counts, but I would like to know your opinion about.

Thanks

d


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