[BioC] why could I only get two different expressed genes by Limma?

yongzi chen chenyongzi77 at yahoo.com.cn
Mon Jun 17 03:31:20 CEST 2013

To whom it may concern.

Hello, I am new in microarray data analysis. recently I want to use Limma to find different expressed genes between grade I and grade 
III, which you could see from the annotation file. but when I use the R 
code below to do this, I only got two different expressed genes, could 
anybody tell me why? Is there anything wrong with my code? thank you in 
advance! and the data is available on line.http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE2990.

Best regards,


Best wishes to you!

Yong-Zi Chen, Ph.D.
Tianjin Cancer Institute
Tianjin Medical University Cancer Institute and Hospital
Huan-Hu-Xi Road, Ti-Yuan-Bei, He-Xi District
Tianjin300060, P.R. China
Phone&Fax:   0086-022-23340123
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