[BioC] Small RNA seq data analysis using DESeq
vfranke at bioinfo.hr
Wed Jun 19 20:44:34 CEST 2013
I have a question regarding the analysis of small RNAseq data using DESeq.
While counting the reads per loci I have weighted the reads by the
reciprocal of the places to which the read maps.
I was wondering whether it is still proper to use the negative binomial
test implemented in DESeq (after rounding the expression estimates) to
determine which loci are differentially expressed?
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