[BioC] help with flowQ vignette

Dan Tenenbaum dtenenba at fhcrc.org
Tue May 14 20:35:32 CEST 2013


Hi Frederico,



On Tue, May 14, 2013 at 11:22 AM, Frederico Moraes Ferreira
<ferreirafm at usp.br> wrote:
> Hi List,
> I'm following the flowQ vignette (DataQualityAssessment.R) and get stucked
> in the transformation bellow.
> I've check manuals, google and ?transformList and can't figure out what's
> wrong.
> Please, could someone give me a hand?
>
>> load("qData.rda")
>> qData[[1]][[1]]
> flowFrame object 'pid02050'
> with 4500 cells and 8 observables:
>         name   desc range minRange maxRange
> $P1    FSC-A  FSC-A  1024        0     1023
> $P2    SSC-A  SSC-A  1024        0     1023
> $P3   FITC-A CD45RA  1024        1    10000
> $P4     PE-A CD45RO  1024        1    10000
> $P5    FL3-A    CD8  1024        1    10000
> $P6 PE-Cy7-A    CD4  1024        1    10000
> $P7    APC-A  CD62L  1024        1    10000
> $P8     Time   <NA>  1024        0     1023
> 82 keywords are stored in the 'description' slot
>> tData <- lapply(qData, function(x) transformList(colnames(x)[3:7], asinh)
>> %on% x)
> Error in FUN(X[[1L]], ...) : could not find function "%on%"

This command works for me. Can you send the output of the
sessionInfo() command (after loading flowQ)? That will help us figure
out the problem.

Thanks,
Dan


>> R.version
>                _
> platform       x86_64-pc-linux-gnu
> arch           x86_64
> os             linux-gnu
> system         x86_64, linux-gnu
> status
> major          3
> minor          0.0
> year           2013
> month          04
> day            03
> svn rev        62481
> language       R
> version.string R version 3.0.0 (2013-04-03)
> nickname       Masked Marvel
>> biocLite()
> BioC_mirror: http://bioconductor.org
> Using Bioconductor version 2.12 (BiocInstaller 1.10.1), R version 3.0.0.
>
>
>
>
> Best,
>
> --
> Dr. Frederico Moraes Ferreira
> University of Sao Paulo
> Heart Institute, School of Medicine
> Laboratoy of Immunology
> Av. Dr. Enéas de Carvalho Aguiar, 44
> 05403-900     Sao Paulo - SP
> Brasil
>
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