[BioC] RTPCR - concentration rather than Ct

[Lavinia Gordon]

Dear all,

I have some rtpcr data where the control genes show considerable differences in Ct values at their two locations (at the start and at the end of the plate).  I've used HTqPCR in the past (thanks Heidi!) with great results.  However in this case I am working with the concentrations (calculating relative telomere length).  Previous papers have simply suggested matching the sample well position on the plates for the telomere and single copy PCRs to give the lowest variability, I wondered if anyone had any other suggestions?
Any advice greatly appreciated,
With regards,


Lavinia Gordon
Senior Research Officer
Quantitative Sciences Core, Bioinformatics

Murdoch Childrens Research Institute
The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia 
T 03 8341 6221
www.mcri.edu.au

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