[BioC] tutorials for RNA-seq analysis in Bioconductor

Steve Lianoglou lianoglou.steve at gene.com
Wed May 22 22:53:14 CEST 2013


Hi,

On Wed, May 22, 2013 at 1:36 PM, Fiona Ingleby <fiona.ingleby at gmail.com> wrote:
> Hi everyone,
>
> Quick question: can anyone recommend tutorials/resources which are available online that I could use to get started with the basics of analysing RNA-seq data in Bioconductor? I've come across a few things (sets of lecture slides and sets of code to work through) but I just thought I would ask if anyone had found anything particularly useful? Ideally I'm wanting to focus on alignment and differential expression analysis, so I'd be grateful if anyone has any recommendations.

Reading through the vignettes for packages built to handle these
things would be a good start, namely:

* easyRNASeq
  http://bioconductor.org/packages/2.12/bioc/vignettes/easyRNASeq/inst/doc/easyRNASeq.pdf

* HTSeqGene
  http://bioconductor.org/packages/2.12/bioc/vignettes/HTSeqGenie/inst/doc/HTSeqGenie.pdf

* QuasR
  http://bioconductor.org/packages/2.12/bioc/vignettes/QuasR/inst/doc/QuasR-Overview.pdf


HTH,
-steve

--
Steve Lianoglou
Computational Biologist
Department of Bioinformatics and Computational Biology
Genentech



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