[BioC] Setting weight to agilent source

Kaj Chokeshaiusaha [guest] guest at bioconductor.org
Sun Oct 13 07:18:07 CEST 2013


Dear R helpers,

I'm very new to Agilent analysis and still on my self-learning the usage of 'limma'. I would like to apologize everyone if my question is too naive to be asked.

I have read 'Using of Limma'. In the topic "4.7 Image-derived Spot Quality Weights", there are examples of setting weights to genepix and spot sources provided.

According to my zero experience, I do have no idea how to do this properly with files of 'agilent' source.

Would you please recommend me some 'should be done' settings? I really need suggestions from experienced people at this step.

Best Regards,
Kaj Chokeshaiusaha

 -- output of sessionInfo(): 

R version 2.15.3 (2013-03-01)
Platform: x86_64-w64-mingw32/x64 (64-bit)

locale:
[1] LC_COLLATE=Thai_Thailand.874  LC_CTYPE=Thai_Thailand.874   
[3] LC_MONETARY=Thai_Thailand.874 LC_NUMERIC=C                 
[5] LC_TIME=Thai_Thailand.874    

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base  

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