[BioC] Error in heatmapSig function

Maria Mengual [guest] guest at bioconductor.org
Thu Oct 31 17:20:23 CET 2013

This is my first time using R and HTqPCR package. I'm trying to do a miRNAs expression analysis. 

I have a problem in the end of the analysis, I want to represent my significant different expression miRNAs in a HeatMap using heatmapSig function, but it isn't work.

These is my error:

heatmapSig(DCtnorm.ttest, comparison = "all", zero.center =  TRUE)
Error in x$ddCt : $ operator is invalid for atomic vectors

Thank you!


 -- output of sessionInfo(): 

R version 3.0.2 (2013-09-25)
Platform: i386-w64-mingw32/i386 (32-bit)

[1] LC_COLLATE=Italian_Italy.1252  LC_CTYPE=Italian_Italy.1252   
[3] LC_MONETARY=Italian_Italy.1252 LC_NUMERIC=C                  
[5] LC_TIME=Italian_Italy.1252    

attached base packages:
[1] parallel  stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
[1] HTqPCR_1.16.0      limma_3.18.1       RColorBrewer_1.0-5
[4] Biobase_2.22.0     BiocGenerics_0.8.0

loaded via a namespace (and not attached):
 [1] affy_1.40.0           affyio_1.30.0         BiocInstaller_1.12.0 
 [4] bitops_1.0-6          caTools_1.16          gdata_2.13.2         
 [7] gplots_2.12.1         gtools_3.1.0          KernSmooth_2.23-10   
[10] preprocessCore_1.24.0 stats4_3.0.2          tools_3.0.2          
[13] zlibbioc_1.8.0       

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