[BioC] phyloseq/DESeq gives negative transformed values

[Sophie Josephine Weiss]

Hello,
I have microbiome data with no replicates, from different conditions.  I am
trying to transform the data using the DESeq method, as described in
McMurdie and Holmes 2014.

The attached file is the definition I am using, as per the supplemental
info in McMurdie and Holmes 2014, and the .biom file I am using.

Thank you for your help,
Sophie