[BioC] Why is the volcano plot from limma package is more beautiful ( symmetrical ) than that from raw t.test calculation?

Sean Davis sdavis2 at mail.nih.gov
Mon Aug 18 03:29:02 CEST 2014


Hi, Allen.

It looks like the scatter plot shows different data for the t-test and for
the limma results.  Perhaps one dataset was normalized and the other was
not or two different normalizations or filters were used?

Sean



On Sun, Aug 17, 2014 at 9:16 PM, my1stbox <my1stbox at 163.com> wrote:

> Dear All,
> Why is the volcano plot from limma package is more beautiful ( symmetrical
> ) than that from raw t.test calculation? Please see attachment for the
> picture.
>
> Best regards,
> Allen
>
> 2014-08-18
>
>
> my1stbox
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