[BioC] Single sample normalization of single-channel Agilent microarrays
Gabriele Zoppoli [guest]
guest at bioconductor.org
Tue Aug 26 18:42:45 CEST 2014
Dear BioConductor community,
when faced with the concept of generating a microarray-based classifier for a clinical condition (say responder vs non-responder to a treatment), I have issues understaing how, after a model is built from a training set, it can be applied prospectively in a serial way in a prospective trial. It is my understanding that most normalization methods depend, at some point, on the information derived from the microarray batch which a given sample is normalized with. Few methods circumvent this issue, such as fRMA (in case one has the possibility to use Affy HGU133 Plus 2.0 arrays) or SCAN.UPC, which would be suitable for most Affy arrays and even dual-channel Agilent arrays. What about single-channel Agilent arrays? And which were the methods used in all the works published before those methods were published? Thanks in advance, I hope this is not too general a question
-- output of sessionInfo():
R version 3.1.0 (2014-04-10)
Platform: x86_64-pc-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=de_BE.UTF-8 LC_COLLATE=en_US.UTF-8 LC_MONETARY=de_BE.UTF-8
[6] LC_MESSAGES=en_US.UTF-8 LC_PAPER=de_BE.UTF-8 LC_NAME=de_BE.UTF-8 LC_ADDRESS=de_BE.UTF-8 LC_TELEPHONE=de_BE.UTF-8
[11] LC_MEASUREMENT=de_BE.UTF-8 LC_IDENTIFICATION=de_BE.UTF-8
attached base packages:
[1] parallel splines stats graphics grDevices utils datasets methods base
other attached packages:
[1] frma_1.16.0 SCAN.UPC_2.6.3 sva_3.10.0 mgcv_1.8-1 nlme_3.1-117 corpcor_1.6.6 foreach_1.4.2
[8] affyio_1.32.0 affy_1.42.3 GEOquery_2.30.1 oligo_1.28.2 Biostrings_2.32.1 XVector_0.4.0 IRanges_1.22.9
[15] oligoClasses_1.26.0 Biobase_2.24.0 BiocGenerics_0.10.0 BiocInstaller_1.14.2 xlsx_0.5.5 xlsxjars_0.6.0 rJava_0.9-6
[22] ggplot2_1.0.0 aod_1.3 survcomp_1.14.0 prodlim_1.4.3 survival_2.37-7 limma_3.20.8
loaded via a namespace (and not attached):
[1] affxparser_1.36.0 bit_1.1-12 bootstrap_2014.4 codetools_0.2-8 colorspace_1.2-4 DBI_0.2-7 digest_0.6.4
[8] ff_2.2-13 GenomeInfoDb_1.0.2 GenomicRanges_1.16.3 grid_3.1.0 gtable_0.1.2 iterators_1.0.7 KernSmooth_2.23-12
[15] lattice_0.20-29 lava_1.2.6 MASS_7.3-33 Matrix_1.1-4 munsell_0.4.2 plyr_1.8.1 preprocessCore_1.26.1
[22] proto_0.3-10 Rcpp_0.11.2 RCurl_1.95-4.1 reshape2_1.4 rmeta_2.16 scales_0.2.4 stats4_3.1.0
[29] stringr_0.6.2 SuppDists_1.1-9.1 survivalROC_1.0.3 tools_3.1.0 XML_3.98-1.1 zlibbioc_1.10.0
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