[BioC] extracting transcripts

Jahn Davik jahn.davik at bioforsk.no
Tue Jan 14 15:17:56 CET 2014


Hi,
In edgeR I do something like this:

lrt<-glmLRT(fit,contrast=c(0,0,0,0,0,0,0,0,-1,1))
topTags(lrt)

Coefficient:  -1*GroupE:Time96 1*GroupJ:Time96 
                     logFC logCPM     LR    PValue       FDR
comp369941_c0_seq1   17.19   9.98 1103.8 4.85e-242 3.05e-237
comp436540_c0_seq6   13.42   3.92  216.0  6.91e-49  2.17e-44
comp438623_c0_seq2  -10.88   5.34  199.7  2.43e-45  5.08e-41
comp437945_c0_seq4  -11.37   4.27  184.9  4.06e-42  6.38e-38
comp437052_c0_seq13 -10.57   6.99  147.1  7.64e-34  9.60e-30
comp434611_c0_seq48 -11.08   3.61  116.7  3.34e-27  3.50e-23
comp423571_c0_seq13 -10.68   2.95  114.3  1.10e-26  9.90e-23
comp419207_c0_seq15  -2.94   6.34  113.6  1.63e-26  1.28e-22
comp430114_c0_seq11  -3.42   6.59   97.3  5.82e-23  3.97e-19
comp432078_c0_seq11 -11.19   4.11   97.2  6.31e-23  3.97e-19


But I would like to extract the, say, 50 top ids or the ids that have an FDR below a certain limit. How do I do that?

Best
Jahn



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