[BioC] Error While running DESeq

Simon Anders anders at embl.de
Sat Jul 5 12:19:58 CEST 2014


Hi Nisha

First the standard advices:

1. Please always post your complete code and the seesion info. Please do 
read the posting guide.

2. Please consider using DESeq2 rather the DESeq.

3. As you do not have replicates, you cannot perform a proper 
statistical analysis anyway.

Now to your data:

4. Your read count table (apart from lacking replication) looks _very_ 
weird. Whenever a gene has more than 2 reads in one of the samples, it 
always has always _exactly_ the same count in both samples! This is a 
impossible to have happened by chance. Something has gone completely 
wrong in creating the table.

So, it's no wonder that the dispersion fitting function gets confused.


   Simon



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