[BioC] how to read FCS3.1 files?

[Mike]

FCS3.1 is supported by the latest flowCore (1.30.7) from bioconductor 
release 2.14 
(http://www.bioconductor.org/packages/release/bioc/html/flowCore.html)

Mike
On 07/07/2014 11:40 AM, mdzunkov at bidmc.harvard.edu wrote:
> Dear Dr. Jiang!
> I am writing you as you are the maintainer of FlowCore and FlowViz R packages.
> I would appreciate your help with my problem:
>
> I changed the FACS machine, now I am using Avalon S3 from BioRad and the software associated to this machine saves the .fcs files in FCS3.1 version only. This is a big problem for me, as my R script is already written for older version FCS3.0 or .lmd files which I was using before. Now I don't know how to load these files in FCS3.1 version. Do you have any idea?
>
>
> This is how my script starts:
>
>> library("flowCore")
>> library("flowViz")
>> PATH<-"."
>> PATTERN<-".fcs"
>> ALTNAM<-TRUE
>> TRANS<-FALSE
>> fs.fast<-read.flowSet(path=PATH, alter.names=ALTNAM, transformation=TRANS, pattern=PATTERN)
> Error in readFCSheader(con) :
>    This does not seem to be a valid FCS2.0 or FCS3.0 file
>
> I use flowViz_1.24.0 and flowCore_1.24.2
>
> Thank you very much!
>
> Maria Dzunkova
>
>
> Department of Gastroenterology
> Beth Israeal Deaconess Medical Center
> Harvard University
> Boston, MA
>
>
>
>
>
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