[BioC] error on detecting regions

Jia, Li (NIH/NCI) [C] li.jia2 at nih.gov
Wed Jun 18 16:31:21 CEST 2014


Hi Pan,

It still has the error message after the update. There are total 80 samples, but I only picked subgroup 42 samples to compare as mentioned in the first email.

> ResultStage <- detectDMR.slideWin(Mset, sampleType=c(postAcute, pre))
Smoothing Chromosome chr1 ...
Smoothing Chromosome chr10 ...
Smoothing Chromosome chr11 ...
Smoothing Chromosome chr12 ...
Smoothing Chromosome chr13 ...
Smoothing Chromosome chr14 ...
Smoothing Chromosome chr15 ...
Smoothing Chromosome chr16 ...
Smoothing Chromosome chr17 ...
Smoothing Chromosome chr18 ...
Smoothing Chromosome chr19 ...
Smoothing Chromosome chr2 ...
Smoothing Chromosome chr20 ...
Smoothing Chromosome chr21 ...
Smoothing Chromosome chr22 ...
Smoothing Chromosome chr3 ...
Smoothing Chromosome chr4 ...
Smoothing Chromosome chr5 ...
Smoothing Chromosome chr6 ...
Smoothing Chromosome chr7 ...
Smoothing Chromosome chr8 ...
Smoothing Chromosome chr9 ...
Error in rowcoltt(x, fac, tstatOnly, 1L) :
  length(fac)=42, ncol(x)=80, should be the same.

> sessionInfo()
R version 3.1.0 (2014-04-10)
Platform: x86_64-apple-darwin10.8.0 (64-bit)

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] parallel  grid      stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
 [1] methyAnalysis_1.6.0                 methylumi_2.10.0                    minfi_1.10.2                        bumphunter_1.4.2
 [5] locfit_1.5-9.1                      iterators_1.0.7                     foreach_1.4.2                       lattice_0.20-29
 [9] matrixStats_0.10.0                  ggplot2_1.0.0                       reshape2_1.4                        scales_0.2.4
[13] FDb.InfiniumMethylation.hg19_2.0.10 BSgenome.Hsapiens.UCSC.hg19_1.3.99  BSgenome_1.32.0                     Biostrings_2.32.0
[17] XVector_0.4.0                       GenomicFeatures_1.16.2              org.Hs.eg.db_2.14.0                 RSQLite_0.11.4
[21] DBI_0.2-7                           AnnotationDbi_1.26.0                Biobase_2.24.0                      GenomicRanges_1.16.3
[25] GenomeInfoDb_1.0.2                  IRanges_1.22.9                      BiocGenerics_0.10.0                 BiocInstaller_1.14.2

loaded via a namespace (and not attached):
 [1] affy_1.42.2              affyio_1.32.0            annotate_1.42.0          base64_1.1               BatchJobs_1.2            BBmisc_1.6
 [7] beanplot_1.1             BiocParallel_0.6.1       biomaRt_2.20.0           biovizBase_1.12.1        bitops_1.0-6             brew_1.0-6
[13] cluster_1.15.2           codetools_0.2-8          colorspace_1.2-4         dichromat_2.0-0          digest_0.6.4             doRNG_1.6
[19] fail_1.2                 Formula_1.1-1            genefilter_1.46.1        GenomicAlignments_1.0.1  genoset_1.16.2           gtable_0.1.2
[25] Gviz_1.8.3               Hmisc_3.14-4             illuminaio_0.6.0         KernSmooth_2.23-12       latticeExtra_0.6-26      limma_3.20.5
[31] lumi_2.16.0              MASS_7.3-33              Matrix_1.1-4             mclust_4.3               mgcv_1.7-29              multtest_2.20.0
[37] munsell_0.4.2            nleqslv_2.2              nlme_3.1-117             nor1mix_1.1-4            pkgmaker_0.22            plyr_1.8.1
[43] preprocessCore_1.26.1    proto_0.3-10             R.methodsS3_1.6.1        RColorBrewer_1.0-5       Rcpp_0.11.2              RCurl_1.95-4.1
[49] registry_0.2             reshape_0.8.5            rngtools_1.2.4           Rsamtools_1.16.1         rtracklayer_1.24.2       sendmailR_1.1-2
[55] siggenes_1.38.0          splines_3.1.0            stats4_3.1.0             stringr_0.6.2            survival_2.37-7          tools_3.1.0
[61] VariantAnnotation_1.10.2 XML_3.98-1.1             xtable_1.7-3             zlibbioc_1.10.0

Thanks

From: Pan Du <dupan.mail at gmail.com<mailto:dupan.mail at gmail.com>>
Date: Tue, 17 Jun 2014 14:40:38 -0700
To: "Jia, Li (NIH/NCI) [C]" <jial2 at mail.nih.gov<mailto:jial2 at mail.nih.gov>>
Cc: "bioconductor at r-project.org<mailto:bioconductor at r-project.org>" <bioconductor at r-project.org<mailto:bioconductor at r-project.org>>
Subject: Re: error on detecting regions

Hi

Please update your R and methyAnalysis package to the latest released versions (R 3.1.0, methyAnalysis 1.6.0), and send me the error messages and related data if it still happens.
Thanks!

Pan



On Tue, Jun 17, 2014 at 2:30 PM, guest [guest] <guest at bioconductor.org<mailto:guest at bioconductor.org>> wrote:
Dear Users,

I met an error that struggled me really long time to figure out. I tried to use detectDMR.slideWin() function, but no matter how I change the factors assign to sampleType, it still has the error.

I have several different conditions in a factor - Stage (PRE, POST-ACUTE, POST-COL and POST-LAT). I would like to compare POST-ACUTE with PRE.

Here are script:
Mset.postAcute=Mset[,Mset$Stage=='POST-ACUTE']
postAcute <- Mset.postAcute$Stage
Mset.pre=Mset[,Mset$Stage=='PRE']
pre <- Mset.pre$Stage

ResultStage <- detectDMR.slideWin(Mset, sampleType=c(postAcute, pre))

Error message:
Error in rowcoltt(x, fac, tstatOnly, 1L) :
  length(fac)=42, ncol(x)=80, should be the same.
In addition: Warning messages:
1: Please use rownames. We are switching away from eSet-specific methods.
2: Please use colnames. We are switching away from eSet-specific methods.
3: Please use rownames. We are switching away from eSet-specific methods.
4: Please use colnames. We are switching away from eSet-specific methods.


 -- output of sessionInfo():

> sessionInfo()
R version 3.0.2 (2013-09-25)
Platform: x86_64-apple-darwin10.8.0 (64-bit)

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] grid      parallel  stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
 [1] FDb.InfiniumMethylation.hg19_2.0.10 BSgenome.Hsapiens.UCSC.hg19_1.3.19  BSgenome_1.30.0                     GenomicFeatures_1.14.5
 [5] methyAnalysis_1.4.2                 org.Hs.eg.db_2.10.1                 RSQLite_0.11.4                      DBI_0.2-7
 [9] AnnotationDbi_1.24.0                lumi_2.14.2                         BiocInstaller_1.12.1                VariantAnnotation_1.8.13
[13] Rsamtools_1.14.3                    Biostrings_2.30.1                   GenomicRanges_1.14.4                XVector_0.2.0
[17] IRanges_1.20.7                      ggplot2_1.0.0                       phyloseq_1.6.1                      picante_1.6-2
[21] nlme_3.1-117                        ape_3.1-2                           ade4_1.6-2                          knitr_1.6
[25] vegan_2.0-10                        lattice_0.20-29                     permute_0.8-3                       biom_0.3.12
[29] metagenomeSeq_1.5.47                devtools_1.5                        gplots_2.13.0                       RColorBrewer_1.0-5
[33] matrixStats_0.10.0                  limma_3.18.13                       Biobase_2.22.0                      BiocGenerics_0.8.0

loaded via a namespace (and not attached):
 [1] affy_1.40.0           affyio_1.30.0         annotate_1.40.1       base64_1.1            beanplot_1.1          biomaRt_2.18.0
 [7] biovizBase_1.10.8     bitops_1.0-6          bumphunter_1.2.0      caTools_1.17          cluster_1.15.2        codetools_0.2-8
[13] colorspace_1.2-4      dichromat_2.0-0       digest_0.6.4          doRNG_1.6             evaluate_0.5.5        foreach_1.4.2
[19] formatR_0.10          Formula_1.1-1         gdata_2.13.3          genefilter_1.44.0     genoset_1.14.0        gtable_0.1.2
[25] gtools_3.4.1          Gviz_1.6.0            Hmisc_3.14-4          httr_0.3              igraph_0.7.1          illuminaio_0.4.0
[31] iterators_1.0.7       itertools_0.1-3       KernSmooth_2.23-12    labeling_0.2          latticeExtra_0.6-26   locfit_1.5-9.1
[37] MASS_7.3-33           Matrix_1.1-4          mclust_4.3            memoise_0.2.1         methylumi_2.8.0       mgcv_1.7-29
[43] minfi_1.8.9           multtest_2.18.0       munsell_0.4.2         nleqslv_2.2           nor1mix_1.1-4         pkgmaker_0.22
[49] plyr_1.8.1            preprocessCore_1.24.0 proto_0.3-10          R.methodsS3_1.6.1     Rcpp_0.11.2           RCurl_1.95-4.1
[55] registry_0.2          reshape_0.8.5         reshape2_1.4          RJSONIO_1.2-0.2       rngtools_1.2.4        rtracklayer_1.22.7
[61] scales_0.2.4          siggenes_1.36.0       splines_3.0.2         stats4_3.0.2          stringr_0.6.2         survival_2.37-7
[67] tools_3.0.2           whisker_0.3-2         XML_3.95-0.2          xtable_1.7-3          zlibbioc_1.8.0


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