[BioC] flowClean

[Kipper Fletez-Brant]

Great, thanks for the update!

On Mon 23 Jun 2014 05:26:21 PM EDT, Justin Meskas wrote:
> Hi Kipper,
>
> I downloaded your update and I ran "clean" on all my data for my current project and everything came out with no errors. Thank you very much for your quick fix.
>
> Justin
>
> ________________________________________
> From: Kipper Fletez-Brant [cafletezbrant at gmail.com]
> Sent: June 21, 2014 6:07 AM
> To: Justin Meskas
> Cc: Ryan Brinkman; bioconductor at r-project.org
> Subject: Re: flowClean
>
> Justin,
>
> Thanks for your patience.  I've fixed this and pushed an update to
> Bioconductor, which should be live tomorrow.
>
> Thanks again,
> Kipper
>
> On Thu 19 Jun 2014 06:41:04 PM EDT, Justin Meskas wrote:
>> Hi Kipper,
>>
>> Thank you for your quick reply. I had previously tried flowClean on uncompensated and untransformed data, and the same error was given.
>>
>> Thanks,
>> Justin
>>
>> ________________________________________
>> From: Kipper Fletez-Brant [cafletezbrant at gmail.com]
>> Sent: June 19, 2014 3:26 PM
>> To: Justin Meskas
>> Cc: Ryan Brinkman; bioconductor at r-project.org
>> Subject: Re: flowClean
>>
>> Hi Justin,
>>
>> I'm cc'ing the Bioc list so that others having this problem might see this.
>>
>> I have seen this error before. While it will take some time to diagnose, one comment I have is that we developed this algorithm to operate on uncompensated data. Try using flowClean before you do any other processing, and let me know how that works.
>>
>> Thanks,
>> Kipper
>>
>> On Jun 19, 2014 5:42 PM, "Justin Meskas" <jmeskas at bccrc.ca<mailto:jmeskas at bccrc.ca>> wrote:
>> Hello Kipper,
>>
>> I am in Dr. Ryan Brinkman's lab and I was trying to use your flowClean package. I have downloaded version 1.0.0 from Bioconductor and I received a similar error for many of my FCS files. I have attached one FCS file and the code I used to get the error. The error was the following:
>>
>> Error in if (x == 0) { : missing value where TRUE/FALSE needed
>>
>> I do not know if there is a problem with my data, or if I am using the data incorrectly with flowClean, but if there is any help you could provide me that would be greatly appreciated.
>>
>> Thank you,
>> Justin