[BioC] HTSeq-Count
Julia [guest]
guest at bioconductor.org
Fri Sep 5 16:41:05 CEST 2014
Hi all,
I am new to the field of seq and performed a RIP-Seq experiment using HTSeq count as counter.
I get now the following (using union, but doesn´t look better for interesection_strict):
__no_feature 1503377
__ambiguous 490772
__too_low_aQual 0
__not_aligned 0
__alignment_not_unique 5277314
When I sum up counts for all genes, I get 3227845.
The number for __no_feature, __ambiguous, __alignment_not_unique look very high.
Does somebody have an idea for that?
(Additional info: We did random priming and mapped with STAR and masked rRNA loci)
Best wishes
Julia
-- output of sessionInfo():
.
--
Sent via the guest posting facility at bioconductor.org.
More information about the Bioconductor
mailing list