[R] Can any one help me on format file data.

Earl F. Glynn efg at stowers-institute.org
Thu Jul 19 00:48:40 CEST 2007

"Horacio Castellini" <horacio9573 at gmail.com> wrote in message 
news:90e13c0707180751y7d32d633m2930731c6f16db24 at mail.gmail.com...
> Hi all.
>           I'd like know what is the format file saved by  Leica
> Microsystems TCS SP2-AOBS equipped with a SP2-FCS2 Leica Microsystems
> workstation its datas. Cause it save in *.fcs extention  file but
> ins't flow cytometry standart format file...

>From a Google search for "SP2-FCS2 Leica Microsystems" I found this paper 
http://jcs.biologists.org/cgi/reprint/118/24/5825.pdf that talked about 
Fluorescence Correlation Spectroscopy (FCS) data.  Is is possible you have 
Fluorescence Correlation Spectroscopy (FCS) data instead of flow cytometry 
(FCS) data?  I've worked some with both types of FCS data.

I wrote a ConfoCor 3 Fluroescence Correlation Spectroscopy FCS viewer in R:
The software that reads that older Fluroescence Correlation Spectroscopy FCS 
data was in Delphi 
It would be a bit of a pain to read that older Fluroescence Correlation 
Spectroscopy FCS bit stream in R.   But it'slikely Leica's format is 
different than Zeiss.

I have also worked with some versions of flow cytometery FCS data (e.g., see 
There are different versions of that FCS standard too.

If you send me a small file, I'll see if I can recognize if it's a format 
I've seen.


Earl F. Glynn
Scientific Programmer
Stowers Institute for Medical Research

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