[R] Re al Time PCR data analysis help

[Steve Lianoglou]

On Jul 20, 2009, at 6:43 PM, Andrej Kastrin wrote:

> Check out qpcR package.
>
> Andrej
>
> gauravbhatti wrote:
>> Hi I need help since I have never worked on RT PCR data before .  I  
>> have 8
>> data files each corresponding  to a single pcr run. Each file also  
>> represent
>> 10 samples(5 cancer and 5 healthy) belonging to a unique gene. I  
>> have 7
>> unique genes and 1 reference (total 8 files). Can any body help me  
>> with
>> preproceesing and normalization?

Also, try asking this question again on the bioconductor list, as  
you'll likely find a higher density of people there doing related work.

Some other packages that might help:

   * http://bioconductor.org/packages/2.4/bioc/html/qpcrNorm.html
   * http://bioconductor.org/packages/2.4/bioc/html/SLqPCR.html

-steve

--
Steve Lianoglou
Graduate Student: Physiology, Biophysics and Systems Biology
Weill Medical College of Cornell University

Contact Info: http://cbio.mskcc.org/~lianos/contact