[R] creating standard curves for ELISA analysis

[aliu]

Hi 1Rnwb,

I am a technical support for Hitachi Solutions and I saw your post regarding
ELISA analysis.  When I opened up your .csv file, I noticed it was a Luminex
file from the Data Collector 1.7 software.  My company developed a multiplex
data analysis software for the Luminex platform called 
http://www.miraibio.com/masterplex-qt/qt-luminex-quantitative-data-analysis-software.html
MasterPlex QT .  I used this software and did ran the curve-fitting for you
and included both the report (.csv file) with the final results and the
MasterPlex QT project file (.mlxq).  Here is one of your standard curves for
"ENA-78(37)":

http://r.789695.n4.nabble.com/file/n3325361/StandardChart.png 

http://r.789695.n4.nabble.com/file/n3325361/ds2_cs_panela_p2_101806.csv
ds2_cs_panela_p2_101806.csv 
http://r.789695.n4.nabble.com/file/n3325361/ds2_cs_panela_p2_101806.mlxq
ds2_cs_panela_p2_101806.mlxq 

I also noticed that quite a few of your samples had pretty low bead counts
(<35) where the data is not significant so they are outlined in the project
file.  I would normally flag these as outliers but I just left all the data
intact.  You'll be able to find this information and much more in my blog
post titled 
http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/ 10 Tips
for Luminex/Bio-Plex Data Analysis .

I hope this information helps.  Feel free to contact me directly at aliu at
miraibio dot com if you have any questions regarding the Luminex data
analysis.

Allen Liu

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