[R] qRT-PCR Sample Maximization software analysis and proper experimental design advice
marcelolaia at gmail.com
Fri May 1 17:00:35 CEST 2015
After a very good advice from here  I am learning about
experimental qRT-PCR design and I found Hellemans et al., 2007 , as
suggested by Jo, and Rieu and Powers 2009 . So, I made a
experimental design for me .
However, I am not sure what software I could use to do the analysis
afterwards. There are qBase+, but we don't have grant to by it. So, we
are try a workaround in R, that is opensource.
Have you any suggestion to me about:
A. My experimental design; http://goo.gl/68h1ul
It is correct? If not, could you suggest me?
B. How I could analyse my experimental desing? What software I could
use? There are R package for that?
I was learning about EasyqPCR package, but, may be it need IRC
(Control) in all run (plate).
Thank you very much!
Marcelo Luiz de Laia
Universidade Federal dos Vales do Jequitinhonha e Mucuri
Laia, M. L.
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