[BioC] Question about MAS5 implementation in Affy package
Rafael A. Irizarry
ririzarr at jhsph.edu
Fri May 16 15:27:04 MEST 2003
On Fri, 16 May 2003 Dapeng.Xu at aventis.com wrote:
> Here is what I run:
>
> > Data <- ReadAffy()
> > eset <- expresso(Data, normalize=FALSE,
> bgcorrect.method="mas",pmcorrect.method="mas",summary.method="mas")
>
>
> The method "mas" should be MAS5 method as it says in the introduction
> document.
the code was written using mas a short-hand for MAS 5.0.
>
> But the output file only has 2 columns: qualifier names and numerical signal
> values.
>
> How do I get absent/present decision calls?
expresso returns expression values not present absent calls.
present/absent calls, as implemented by Affymetrix is
not currently implemeneted. however, its relatively easy to code if you
have the MAS 5.0 manual.
>
> Dapeng
>
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