[BioC] How package I use for verify differentialy expressed genes after Normalization with marrayNorm?

Marcelo Luiz de Laia mlaia at fcav.unesp.br
Mon Nov 3 22:20:13 MET 2003


Thanks Jean.

> -----Original Message-----
> From: Jean Yee Hwa Yang [mailto:jean at biostat.ucsf.edu]

(...)

> That is fine,  maM(ka.susc.norm) will return normalized log-ratios =
> log(Cy5 / cy3) which is log(Treated/control).

Hummm! When I typed maM(ka.susc.norm) I see a matrix with 4 colunms x 7680
rows. (Please, see it below).

But, my genes are duplicated in the nylon filter membrane!

Then, I should have only the half (3840 genes).

Which will my error be?

Thanks

Marcelo
++++++++++++

maM(ka.susc.norm) return

       Susc_01.dats Susc_02.dats Susc_03.dats

   [1,] -0.4164532   -0.6577436            NA
   [2,]  6.0630024    2.1253564    -0.8368827
   [3,]  3.7762845    1.6976429    -1.2527906
  (...)
  [10,]         NA           NA            NA
  (...)
[7678,]  2.7573818    3.3141e+00    0.6502689
[7679,]         NA            NA           NA
[7680,]         NA            NA           NA

The last two rows is Zero(0), because it is blank (spoted water without
DNA). I have 530 blanks in my array.
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