[BioC] Estrogen Data
Laurent Gautier
laurent at cbs.dtu.dk
Sun Nov 30 22:04:47 MET 2003
On Sun, Nov 30, 2003 at 09:50:07PM +0100, Bernd Holleczek wrote:
> Dear experts,
>
> I am a beginner in working with R/Bioconductor. At the moment I try to get
> familiar with microarray analysis.
>
> When loading the Estrogen-Data into an AffyBatch, the number of probes of an
> array is 640*640=409600.
> If I calculate the overall amount of pm and mm-values for all probesets of
> an array, I get as result 2*201800=403600.
>
> What is an explanation for this difference or what are the other probes
> necessary for?
There are probes that are not PMs nor MMs. For what we know, some of them are
used to align a grid during the image analysis step. A cute
example dealing with these "unknown" probes can be found in an article about
the affy package (Bioinformatics, in press).
Hopin' it helps,
L.
>
> I'll appreciate suggestions on this question.
> Thanks.
>
> Regards,
>
> Bernd Holleczek
>
> --
> Bernd Holleczek <bernd.holleczek at gmx.de>
>
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