[BioC] rlm function
SERGI VIVES CIVIT
svives at ub.edu
Fri Apr 9 20:13:06 CEST 2004
I'm trying to use rlm function in an unequally replicated 2x3 factorial
combinations of 3 temperatures by 2 amounts.(cDNA microarrays)
For each gene I have 6 log ratios,
and can do a standard factorial experiment analysis, giving a grand
mean, a main effect of amount, and 2 degrees of
freedom for the main effect of temperature, a linear effect and a quadratic
These are the main effects, and the two
degrees of freedom interaction effects, see matrix below.
Here is a little matrix of coefficients for all these terms.
mean + + + + + +
amount - + - + - +
linear temp - - 0 0 + +
amount temp + + -2 -2 + +
amount.linear + - 0 0 - +
amount.quad - + 2 -2 - +
This becomes and orthogonal transformation of the 6 observations by
normalizing the rows appropriately.
Could you please suggest me how to include this matrix design into rlm function?
Thanks in advance.
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