[BioC] Two rma questions

Simon Kidd kidd at mail.rockefeller.edu
Thu Apr 15 16:42:06 CEST 2004

At 8.50am -0400 15/4/04, James MacDonald wrote:
>For your first question, it is almost always preferable to run rma on
>all samples in a set together rather than in two batches. For the second
>question, the bug only concerns justRMA and justGCRMA, so rma and gcrma
>are not affected.

Thanks, however my collaborator thinks I did not phrase my question 
very well and did not emphasis enough that the genetic background of 
the strains could be very different, so this is what she thinks the 
question should be:

>We have performed two experiments. One compares fly strain A with 
>mutation M to fly strain A with a duplication for the wild type gene 
>M. The second experiment compares fly strain B with the same 
>mutation M with a different duplication for the wild type gene M. 
>Aside from the particular mutation we are assaying the genetic 
>background of the strains could be very different.  If one 
>normalizes  arrays from different tissues using dChip, it is 
>suggested that the arrays get normalized separately. Is this also 
>true for RMA or should they be normalized together?

If the strains are very different should they still be normalised together?

Thanks again.


More information about the Bioconductor mailing list