[BioC] correcting for 3' bias in highly amplified RNA samples and Affyme trix chips

Wittner, Ben Wittner.Ben at mgh.harvard.edu
Wed Dec 1 20:49:08 CET 2004

Is there some way in Bioconductor to correct for 3' bias in
Highly amplified RNA samples for Affymetrix chips.

I know that Affy now has a 3' biased human chip, but I have human data that was
done with another chip and cannot be re-done. I also have mouse data and I don't
think there is a 3' biased mouse chip from Affy.

I've heard of people doing such a correction (not necessarily using BioC or R),
but I can't find any mention of it in the affy documentation or in the
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