[BioC] Varying intensity histograms for GCRMA
harry at biochemistry.ucl.ac.uk
Wed Jun 2 10:34:01 CEST 2004
I am comparing results from the 1.4 Bioconductor release
version of GCRMA with the old release 1.3 version.
Plotting the density of expression values results in a
bi-modal distribution in both versions. However, with the old
version the largest peak was at low intensities and there was a
smaller (~1/3) peak at higher intensities. But with the new version,
the largest peak is at high intensities and the smaller peak is at
Biologically, the old results make much more sense (only
about 1/3 of the genes should express in the spinal cord). What has
happened to the code of GCRMA to make the differences?
Best wishes and thanks to all involved in gcrma.
Dr Andrew Harrison Tel: 44 (0) 207 679 3890
Biomolecular Structure and Modelling Unit Fax: 44 (0) 207 679 7193
Biochemistry and Molecular Biology Dept.
University College London
Gower Street Email: harry at biochem.ucl.ac.uk
London, WC1E 6BT, UK http://www.biochem.ucl.ac.uk/~harry
More information about the Bioconductor