[BioC] large amount of slides

Vada Wilcox v_wilcox at hotmail.com
Fri Jun 4 17:06:14 CEST 2004

Dear all,

I have been using RMA succesfully for a while now, but in the past I have 
only used it on a small amount of slides. I would like to do my study on a 
larger scale now, with data (series of experiments) from other researchers 
as well. My questions is the following: if I want to study, let's say 200 
slides, do I have to read them all into R at once (so together I mean, with 
read.affy() in package affy), or is it OK to read them series by series (so 
all wild types and controls of one researcher at a time)?

If it is really necessary to read all of them in at one time how much RAM 
would I need (for let's say 200 CELfiles) and how can I raise the RAM? I now 
it's possible to raise it by using 'max vsize = ...' but I haven't been able 
to do it succesfully for 200 experiments though. Can somebody help me on 

Many thanks in advance,




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