[BioC] affy 1 cycle vs 2 cycle labeling kit

YUK FAI LEUNG yfleung at mcb.harvard.edu
Fri Jun 11 16:56:39 CEST 2004

Hi there,

I wonder if anyone have compared the data correlation between the same 
sample labeled by Affy's one cycle labeling and two cycle labeling kit? 
The amount of some of my pooled samples is in the lower end of the one 
cycle labeling kit. Life would be easier if I use the two cycle kit. But 
as we all know, more processing steps and amplication may lead to 
variation. I am not sure whether I want to use the two cycle kit or just 
collect more samples. Any thoughts?

Yuk Fai Leung
Department of Molecular and Cellular Biology
Harvard University
BL 2079, 16 Divinity Avenue
Cambridge, MA 02138
Tel: 617-495-2599
Fax: 617-496-3321
email: yfleung at mcb.harvard.edu; yfleung at genomicshome.com
URL: http://genomicshome.com

More information about the Bioconductor mailing list