[BioC] Loess normalization for Agilent chips

A.J. Rossini rossini at blindglobe.net
Fri Sep 17 21:53:23 CEST 2004


Why not just use Limma and the corresponding tools?

It's FAR easier that way.   That way you actually get what you pay
for, rather than far less...



"Malard, Joel M" <JM.Malard at pnl.gov> writes:

> I am struggling to get data from Agilent cDNA arrays into BioConductor.
> It seems to me much easier to get the data in affy's normalize.loess()
> than in the other cDNA array packages. 
>
> Given that "one who get a bargain get what he pays for", does anyone has
> comments, recommendations or warnings to share about using an Affy
> normalization procedure on cDNA data?
>
> Thanks,
>
> Joel M. Malard, Ph.D.
> Scientist IV
> Pacific Northwest National Laboratory
> Battelle Boulevard, PO Box 999
> Mail Stop K1-85
> Richland, WA 99352
>
> "I love the audacity of those who have everything to loose from it; the
> moderation of those who have nothing to gain from it." Rostand, Jean
> (1894-1977)
>
>
>
> 	[[alternative HTML version deleted]]
>
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-- 
Anthony Rossini			    Research Associate Professor
rossini at u.washington.edu            http://www.analytics.washington.edu/ 
Biomedical and Health Informatics   University of Washington
Biostatistics, SCHARP/HVTN          Fred Hutchinson Cancer Research Center
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