[BioC] Extracting normalized intensities

Jean Yee Hwa Yang jean at biostat.ucsf.edu
Fri Sep 24 21:32:38 CEST 2004


Hi Gene,

maM extrat the normalized log-ratios.  If you would like to obtain
normalized single channel log-intensities.  You can simply solve the
equations for R and G.

M = (R - G)
A = (R + G) / 2

Jean

On Fri, 24 Sep 2004, Gene Cutler wrote:

> I'm a little unclear on how to extract normalized intensities from a 
> marrayNorm object.  The intensities in maA appear to be the original 
> un-normalized intensities.  There are normalization factors in maMloc 
> and maMscale, but I don't know how to apply them.  The maMscale look 
> like multiplicative values while the maMloc look additive.
> 
> Thanks.
> 
> 
> 
> Gene Cutler
> Research Investigator
> Bioinformatics
> Amgen SF
> 
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