[BioC] Time course experiment
naomi at stat.psu.edu
Wed Dec 7 18:10:13 CET 2005
This is a 1-way ANOVA and can readily be handled using the limma
package in Bioconductor.
I suggest that you read through the limma documentation, trying out
each example using your own data at each step.
If you have Affy data, you will need to normalize the data in Affy
first. Otherwise, you can go straight to limma.
p.s. There must be many people who could help you ad Cold Spring Harbor.
At 04:33 PM 12/6/2005, Khan, Sohail wrote:
>I have a time course experiment with three time points and 5
>replicates for each time point. I want to get an expression
>profile from this data, and get the differential expression for
>different time points. I am new at this so please disregard the
>trivial nature of this question. How can I analyze this type of data?
>COLD SPRING HARBOR LABORATORY
>Genome Research Center
>500 Sunnyside Boulevard
>Woodbury, NY 11797
>Bioconductor mailing list
>Bioconductor at stat.math.ethz.ch
Naomi S. Altman 814-865-3791 (voice)
Dept. of Statistics 814-863-7114 (fax)
Penn State University 814-865-1348 (Statistics)
University Park, PA 16802-2111
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