[BioC] Time course experiment

Naomi Altman naomi at stat.psu.edu
Wed Dec 7 18:10:13 CET 2005

This is a 1-way ANOVA and can readily be handled using the limma 
package in Bioconductor.

I suggest that you read through the limma documentation, trying out 
each example using your own data at each step.

If you have Affy data, you will need to normalize the data in Affy 
first.  Otherwise, you can go straight to limma.


p.s. There must be many people who could help you ad Cold Spring Harbor.

At 04:33 PM 12/6/2005, Khan, Sohail wrote:
>Dear List,
>I have a time course experiment with three time points and 5 
>replicates for each time point.  I want to  get an expression 
>profile from this data, and get the differential expression for 
>different time points.  I am new at this so please disregard the 
>trivial nature of this question.  How can I analyze this type of data?
>Sohail Khan
>Scientific Programmer
>Genome Research Center
>500 Sunnyside Boulevard
>Woodbury, NY 11797
>Bioconductor mailing list
>Bioconductor at stat.math.ethz.ch

Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111

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