[BioC] reading arrays image analysed with spot

[Tom Price]

Auer Michael wrote:

>Hi
>
>I am starting to work with cDNA chips from array comparative
>hybridization. I have severel arrays image analysed with spot 2.0, but the
>parameters (Excel Columns reported) are quite different from the ones in
>swirl.spot in the marray package, which has the command read.Spot. There
>are the following parameters in the Excel sheet. Can anybody help me
>further on how to read them in in a proper way? I would like to normalize
>them in different ways, but first I need to have the two channels in R.
>Furthermore the genes were spotted in triplicates. Is there a way to tell
>that in marray or any other package too?
>Thank you very much
>
>Arr-rowy
>Spot-colx
>Spot-rowy
>Flag
>nfore
>nback
>DapiFore
>DapiBack
>Dapi
>TestFore
>TestBack
>Test
>RefFore
>RefBack
>Ref
>Log2Rat
>RawRat
>SpotCorr
>MinF-B
>Slope
>Log2Slope
>TestIntercept
>RefIntercept
>OriginDist
>MeanLog2Rat
>MeanRat
>MedianRatio
>Log2MedRat
>
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>  
>