[BioC] GC-RMA - interpretation of the expression levels

Sean Davis sdavis2 at mail.nih.gov
Fri Jun 10 14:38:23 CEST 2005


On Jun 10, 2005, at 8:26 AM, Pintilie, Melania wrote:
>
> For example I know that the expression levels (not logged) obtained 
> using
> cDNA microarray can be interpreted (ideally)as: 1=no expression, >2 
> over
> expression and <0.5 under expression.
>

Your best bet is to use statistical methods to determine expression in 
1 group of experiments relative to another.  Look at the vignette for 
multtest and the user guide for Limma 
(http://bioinf.wehi.edu.au/limma/usersguide.pdf) for examples.

Sean



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