[BioC] 5 genes with 4 diff. groups, Howmany Cells needs to be extracted and do LIMMA?
naomi at stat.psu.edu
Thu Jun 23 21:54:38 CEST 2005
You could use limma for this experiment but since you cannot use the eBayes
procedure with so few genes you might be better off using a simpler
statistical package such as SAS or MINITAB.
The routine you want is called one-way ANOVA.
Surely you do not intend to use microarrays with so few genes. This cannot
You need to have biological replication within group to do the analysis you
At 01:31 PM 6/23/2005, Saurin Jani wrote:
>Please guide me in experient..Howmany Cells I need to
>collect in order to be confident in my LIMMA
>statistical analysis? I think LIMMA is the good
>approach because I hvae more than 2 conditions. Please
>see below information.
>I would like to perform experient of 5 genes with 4
>different groups of cells. ( Small scale array)
> GeneA GeneB GeneC GeneD GeneE
>How can I define Design Matrix for these 4 different
>groups of cells?
>Can I do:
>Group1-Group2, Group1-Group3, Group1-Group4
>I would like to study on SMALL scale Array...Is there
>any approach which I could seek?
>Please, let me know if anybody has performed such
>design or analysis?
>Thank you so much in advance,
>Bioconductor mailing list
>Bioconductor at stat.math.ethz.ch
Naomi S. Altman 814-865-3791 (voice)
Bioinformatics Consulting Center
Dept. of Statistics 814-863-7114 (fax)
Penn State University 814-865-1348 (Statistics)
University Park, PA 16802-2111
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