[BioC] Annalyzing replicates in the Agilent chip 4 x44 with limma

Ester Feldmesser ester.feldmesser at weizmann.ac.il
Wed Jul 11 15:14:54 CEST 2007


Hi everybody,
 
The human Agilent chip 4x44 has most of the probes only once, but some 
of then appear in 10 replicates. These replicates are genes and not 
controls.
I am using the Limma package to analyze them. I would like to define 
these replicates as technical ones and leave the other probes (those 
that appear once) as they are.
What is the best way to define only part of the spots as replicates?

Thanks for your help
Esti
-- 

Ester Feldmesser, Ph.D.
Bioinformatics Unit, Department of Biological Services
Weizmann Institute of Science
Levine Building, Room 110
phone: +972-8-934-2614
email: ester.feldmesser at weizmann.ac.il



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