[BioC] Biological replicates

[Yogi Sundaravadanam]

>This is exactly what the t-test is all about.  If you want to state 
that a gene differentially expresses between 2 conditions, don't you 
mean that the difference in expression is higher than the difference 
between biological replicates of the same condition?
 
I was just wondering what I should do if the difference of expression exists between the replicates itself... won't that skew the data a bit?  


 -----Original Message-----
From: Naomi Altman [mailto:naomi at stat.psu.edu] 
Sent: Friday, 28 September 2007 1:01 AM
To: Yogi Sundaravadanam
Subject: Re: [BioC] Biological replicates

This is exactly what the t-test is all about.  If you want to state 
that a gene differentially expresses between 2 conditions, don't you 
mean that the difference in expression is higher than the difference 
between biological replicates of the same condition?

--Naomi

At 01:13 AM 9/27/2007, you wrote:
>Hi all
>
>
>
>I am working with biological replicates and I am a bit worried about the
>biological variation between samples.
>
>For example, the abundance of a certain gene in sample 1 could be
>hundreds of time higher or lower than in sample B. If this is the case,
>
>this will significantly affect the P-value in the t-test.
>
>
>
>As such, my question is whether there is a way we can account for this
>fact in the statistical analysis?
>
>
>
>I will be much grateful if you guys could shed some light on this topic?
>
>
>
>
>Thank you
>
>Yogi
>
>
>
>
>
>
>
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Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111