[BioC] Ringo/Starr getProfiles function
Noah Dowell
noahd at ucla.edu
Fri Dec 11 03:45:21 CET 2009
Hello All,
Another problem has come up that hopefully someone can provide some assistance with...
I am trying to plot ChIP data in relation to the TSS as show in the Starr vignette but the following error comes up when I call the getProfiles function:
> profile = getProfiles(wtNucRatio, probeAnnot, tssAnno, 800, 800, feature = "TSS", borderNames = "TSS", method = "basewise")
chromosome NA Error in probeAnno[paste(chr[i], "index", sep = ".")] :
No mapping 'NA.index' in this 'probeAnno' object.
My particular probeAnno object (probeAnnot) was created using both functions recommended on this board and in the vignette. For example:
using the Ringo function of: posToProbeAnno
> probeAnnot <- posToProbeAnno(pos, genome="Saccer", microarrayPlatform = "Affymetrix")
or the Starr function of bpmapToProbeAnno
> probeAnno = bpmapToProbeAnno(bpmap)
both methods print out the probeAnno mapping for chromosome and do not throw any errors so I assumed the object was made correctly.
So I think the error above may be coming from elsewhere but I cannot find it and do not know how to fix it. Any help would be greatly appreciated.
PS. I created the tssAnno using biomaRt and everything in that looks to be okay...
noah
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