[BioC] reverse complement or no reverse complemnt on biomaRt / biomart.org
James W. MacDonald
jmacdon at med.umich.edu
Tue Oct 13 19:59:58 CEST 2009
Tefina Paloma wrote:
> I apologize for giving such a vague description.
> If I go to the ensemble page, click on "Mine Ensembl with BioMart", chose as
> database ensembl 56, Homo Sapiens, GRCh37, filter by ENST00000280193, click on
> results, and then click on the ENST00000280193 which leads to the transcript
> summary of this ensembl. Then, on the left hand side, I click on "export data"
> and I chose "fasta format", "feature strand", "flanking sequence" and "5utr".
> By doing this, I get the sequences I posted.
That looks like a bug in that export wizard. It doesn't matter which
strand you choose for the 5' UTR, you get the (correct) reverse strand
However, if you choose either feature strand or reverse strand, you get
the forward strand (the reverse complement of the correct 5' flanking
region). If you choose forward strand, you still get the forward strand,
but it is off the 3' end of the gene.
Try pasting this into blat (http://genome.ucsc.edu/cgi-bin/hgBlat) to
see what I mean.
So the only correct results here are from biomaRt.
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James W. MacDonald, M.S.
University of Michigan
Department of Human Genetics
1241 E. Catherine St.
Ann Arbor MI 48109-5618
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