[BioC] paired microarray samples

Naomi Altman naomi at stat.psu.edu
Sat Oct 17 04:51:50 CEST 2009


Have a look at the randomized complete block example.  Donor is a block.

--Naomi

At 03:05 PM 10/16/2009, Chris Fjell wrote:
>I've a question on how to properly deal with paired samples in limma.
>
>For example, I've got an experiment with 3 blood donors (D1, D2, D3) and
>4 treatments (T1, T2, T3, T4) of the blood cells. One single-channel
>array is done for each donor-treatment (e.g. D1 cells treated with T2).
>
>In the past I've treated these as simple biological replicates, so the
>code flow is like:
>
>treatments=pData(BSData.quantile)$Sample_Group # T1, T2, T3, T4,  from
>Illumina beadarray data
>treatments=as.factor(treatments)
>design=model.matrix(~0+treatments)
>colnames(design)=levels(treatments)
>fit = lmFit(exprs(BSData.quantile), design)
>cont.matrix = makeContrasts(T1vT2 = T1 - T2, T3vT1 = T3 - T1, levels =
>design)
>fit = contrasts.fit(fit, cont.matrix)
>ebFit = eBayes(fit)
>
>for( comp in colnames( cont.matrix ) ) {
>     tt = topTable(ebFit, coef = comp)
>     ...
>     [write results to file...]
>}
>
>
>But donors are responding quite differently so I don't want to simple
>pool all the treatments, I want to consider response of each donor, i.e.
>"Paired Samples".
>
>
>
>I found this snippet on the BioC newsgroup at
>https://stat.ethz.ch/pipermail/bioconductor/2006-May/012969.html
>Calculate the logFC ratios manually per donor, then pass it to lmFit() -
>seems like the standard paired t-test.
>
>For my example, for the comparison of T1 to T2
>exM = exprs(BSData.quantile)
>diffD1 = exM[, which( donors == "D1" & samples == "T1" )] - exM[, which(
>donors == "D1" & samples == "T2" )]
>diffD2 = exM[, which( donors == "D2" & samples == "T1" )] - exM[, which(
>donors == "D2" & samples == "T2" )]
>diffD3 = exM[, which( donors == "D3" & samples == "T1" )] - exM[, which(
>donors == "D3" & samples == "T2" )]
>
>paired_ex = cbind( diffD1, diffD2, diffD3)
>fit = lmFit(paired_ex)
>
>The example cited above also uses "block = origin, correlation =
>dupcor$cons" parameters to lmFit() but this is for technical replicates (?)
>This works but I get dramatically worse p-values (due I assume to using
>half the  number of "samples": 3 ratios tested against 0 versus testing
>two groups of 3).
>
>
>But the limma user's guide (Oct 22, 2008 version, Chapter 8, p.40) gives
>another example, using model.matrix() not makeContrasts():
>
> > SibShip <- factor(targets$SibShip)
> > Treat <- factor(targets$Treatment, levels=c("C","T"))
> > design <- model.matrix(~SibShip+Treat)
> > fit <- lmFit(eset, design)
> > fit <- eBayes(fit)
> > topTable(fit, coef="TreatT")
>
>I do the similar thing for my data ...
>
>design_paired_treatments = model.matrix( ~donors+treatments )
>fit_ps = lmFit( exprs(BSData.quantile),design_paired_treatments)
>fit_ps = eBayes( fit_ps )
>
>and look at the columns of my design matrix and it's like
>colnames( design_paired_treatments )
>[1] "(Intercept)"    "donorsD1"       "donorsD2"       "treatmentsT1"
>[5] "treatmentsT2"  "treatmentsT3"
>
>Donor D3 and treatment T4 don't appear in the design.
>
>and I get some results...
>tt = topTable( fit_ps, coef="donorsD1")
>tt
>           ID logFC AveExpr      t      P.Value    adj.P.Val    B
>  6370315 -6.58    8.74      -119.4  4.26e-17   2.08e-12    17.6
>  7160474  3.59    8.77       56.5     7.43e-14   1.37e-09    16.6
>  5260484 -5.69    8.71      -55.8     8.39e-14  1.37e-09    16.6
>
>This is a regression model, I think...  I can see why the two terms
>(donor D3 and treatment T4) disappeared - they have to be restated in
>terms of the rest, if I remember right for doing ANOVA with indicator
>variables.
>
>
>
>Is there a recommended way to do this with limma? Or another package?
>
>Thanks for any advice.
>
>-Chris
>
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Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111



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