[BioC] Imitating Affy's own steps

James W. MacDonald jmacdon at med.umich.edu
Mon Apr 12 15:06:26 CEST 2010



Timothy Wu wrote:
> On Mon, Apr 5, 2010 at 10:24 PM, James W. MacDonald
> <jmacdon at med.umich.edu>wrote:
> 
>> Hi Timothy,
>>
>> AFAIK, you have two choices. Using the affy package, you can use mas5() and
>> mas5calls(). You can also use justMAS() from the simpleaffy package.
>>
>> I don't believe either one will give you exactly the same results. For the
>> affy package see
>>
>> http://bmbolstad.com/misc/MAS5diff/Mas5difference.html
>>
>> for an explanation of the differences.
>>
>> Best,
>>
>> Jim
>>
>>
> Thanks for the reply. It looks pretty close.
> 
> So is the expresso() command listed on the page
> *
> eset <- expresso(Data,
> bgcorrect.method="mas",normalize=FALSE,pmcorrect.method="mas",summary.method="mas")
> *
> 
> be the same as the justMAS()?

Well, the intent is the same, but the implementation is different, so I 
wouldn't be surprised if there were differences there as well.

Best,

Jim


> 
> Thanks in advance.
> 
> Timothy
> 
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> 
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-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826
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