[BioC] Fwd: microarray data without replication

[Richard Pearson]

Hi Ina

You've not said what microarrays you are using or what type(s) of analysis you want to do. 
If you're wanting to look for differentially expressed genes from Affymetrix arrays, both 
bgx and puma can make use of technical error from single arrays per condition. The bgx 
folks wrote a paper about doing this:

http://www.biomedcentral.com/1471-2105/7/353/abstract/

Of course, this is no substitute for using biological replicates...

Best wishes

Richard


Ina Hoeschele wrote:
> 
> ----- Forwarded Message -----
> From: "Ina Hoeschele" <inah at vbi.vt.edu>
> To: bioconductor at stat.math.ethz.ch
> Sent: Monday, May 24, 2010 5:07:54 PM
> Subject: microarray data without replication
> 
> Hi,
> is there an R package (or some other software) for analyzing microarray
> data without replication? I have written my own code (essentially based
> on ASE and data permutation) but am still wondering whether some other
> software exists that I could try (I'm finding a few genes but not too
> much). Thanks, Ina
> 
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-- 
Dr Richard D Pearson                       richard.pearson at well.ox.ac.uk
Wellcome Trust Centre for Human Genetics   http://www.well.ox.ac.uk/~rpearson
University of Oxford                       Tel: +44 (0)1865 617890
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