[BioC] normalization one color microarrays

Sean Davis sdavis2 at mail.nih.gov
Wed Aug 24 23:04:30 CEST 2011


On Wed, Aug 24, 2011 at 4:08 PM, Paz Tapia Ramirez
<verotapia at alumnos.utalca.cl> wrote:
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> Hello, I am starting to work with ONE color microarrays from Agilent, and I read that during the pre-processing is main tasks, such as background adjustment, normalization and summarization. Currently I have corrected the background and  normalized samples, but do not understand the step of the summarization. Can you help me please?
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Hi, Paz.

For Agilent, there is not typically a summarization step (pulling
multiple probes into a "probe set").  Each probe is designed to
measure nucleotides independently.

Sean



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